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tev protease site  (Addgene inc)


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    Structured Review

    Addgene inc tev protease site
    Tev Protease Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tev+protease+site/pET+His6+LIC+cloning+vector+(2Bc-T)+(Plasmid+%2337236)/bio_rxiv__64898__2026__03__25__714350-229-14-18
    Average 93 stars, based on 19 article reviews
    tev protease site - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Inner membrane components of the plasmid pKM101 type IV secretion system TraE and TraD are DNA-binding proteins
    Article Snippet: pHT TraD , Kan R ; T7 promoter vector for the expression of 6xHis-tagged full-length domain of pKM101 TraD , . .. pMSP1D1 , Kan R ; pET28a derivate containing mutant (1–11) of MSP1; His-tag on N-terminus followed by spacer sequence and TEV protease site , Addgene #20,061. .. pMSP1E3D1 , Kan R ; pET28a derivate containing extended MSP1D1; contains repeats of helices 4, 5 and 6; N-terminal 7-his tag followed by spacer sequence and TEV protease cleavage site , Addgene #20,066.

    Sequencing:

    Article Title: Inner membrane components of the plasmid pKM101 type IV secretion system TraE and TraD are DNA-binding proteins
    Article Snippet: pHT TraD , Kan R ; T7 promoter vector for the expression of 6xHis-tagged full-length domain of pKM101 TraD , . .. pMSP1D1 , Kan R ; pET28a derivate containing mutant (1–11) of MSP1; His-tag on N-terminus followed by spacer sequence and TEV protease site , Addgene #20,061. .. pMSP1E3D1 , Kan R ; pET28a derivate containing extended MSP1D1; contains repeats of helices 4, 5 and 6; N-terminal 7-his tag followed by spacer sequence and TEV protease cleavage site , Addgene #20,066.

    Article Title: Inner membrane components of the plasmid pKM101 type IV secretion system TraE and TraD are DNA-binding proteins
    Article Snippet: pMSP1E3D1 , Kan R ; pET28a derivate containing extended MSP1D1; contains repeats of helices 4, 5 and 6; N-terminal 7-his tag followed by spacer sequence and TEV protease cleavage site , Addgene #20,066. .. pMSP2N2 , Kan R ; pET28a derivate containing tandem MSP; His-tag on N-terminus followed by spacer sequence and TEV protease site , Addgene #29,520. ..

    Plasmid Preparation:

    Article Title: Selective BET bromodomain inhibition as an antifungal therapeutic strategy
    Article Snippet: Proteins were concentrated to >20 mg ml −1 on a Centricon device (Millipore) and used for ITC, TSA and crystallization experiments. .. Human SMARCA2 and SMARCA4 BD plasmids bearing an N-terminal His tag and TEV protease site were purchased from Addgene (plasmid no. 73250 and 74664, respectively). ..

    Article Title: Apical Localization of RNA Polymerases Modulate Transcription Dynamics and Supercoiling Domains Revealed by Cryo-ET
    Article Snippet: The gene fragment expressing the reverse gyrase (TopR2) was obtained by PCR from Sulfolobus solfataricus genomic DNA (ATCC, 35092D-5). .. This fragment was inserted into an expression plasmid with His6 N-terminal tag with a TEV protease site (2Bc-T, Addgene #37236) using ligation independent cloning. ..

    Expressing:

    Article Title: Apical Localization of RNA Polymerases Modulate Transcription Dynamics and Supercoiling Domains Revealed by Cryo-ET
    Article Snippet: The gene fragment expressing the reverse gyrase (TopR2) was obtained by PCR from Sulfolobus solfataricus genomic DNA (ATCC, 35092D-5). .. This fragment was inserted into an expression plasmid with His6 N-terminal tag with a TEV protease site (2Bc-T, Addgene #37236) using ligation independent cloning. ..

    Ligation:

    Article Title: Apical Localization of RNA Polymerases Modulate Transcription Dynamics and Supercoiling Domains Revealed by Cryo-ET
    Article Snippet: The gene fragment expressing the reverse gyrase (TopR2) was obtained by PCR from Sulfolobus solfataricus genomic DNA (ATCC, 35092D-5). .. This fragment was inserted into an expression plasmid with His6 N-terminal tag with a TEV protease site (2Bc-T, Addgene #37236) using ligation independent cloning. ..

    Cloning:

    Article Title: Apical Localization of RNA Polymerases Modulate Transcription Dynamics and Supercoiling Domains Revealed by Cryo-ET
    Article Snippet: The gene fragment expressing the reverse gyrase (TopR2) was obtained by PCR from Sulfolobus solfataricus genomic DNA (ATCC, 35092D-5). .. This fragment was inserted into an expression plasmid with His6 N-terminal tag with a TEV protease site (2Bc-T, Addgene #37236) using ligation independent cloning. ..



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